Characterization of Trypanozoon stocks by isoelectric focusing and isoenzyme analysis.
نویسندگان
چکیده
Isoelectric focusing on ultrathin polyacrylamide foils, coupled with isoenzymatic analysis using five different enzymes, was used to characterize blood-forms of T.b. brucei, T.b. rhodesiense and T.b. gambiense and procyclic forms of T.b. brucei and T.b. rhodesiense. The protein concentrations in the lysates were quantified. Qualitative as well as quantitative differences were found.
منابع مشابه
Characterization by isoenzyme electrophoresis of Trypanozoon stocks from sleeping sickness endemic areas of south-east Uganda.
An epidemic of sleeping sickness, which started in 1976 in a focus within the county of Luuka in Central Busoga, has spread to cover the three districts of Busoga and large parts of the neighbouring districts of Tororo and Mukono. Forty-three isolates of the subgenus Trypanozoon from Busoga and Tororo (27 from man, 9 from cows, 2 from pigs and 5 from tsetse flies) were compared by thin-layer st...
متن کاملIsoelectric Focusing and PCR-RFLP Joined Techniques for Alpha1-antitrypsin Deficiency Detection
53 persons suspected to alpha1-antitrypsin deficiency detection (AATD) were investigated for ZZ, MZ, ZS, SS, and MS alleles analysis by serum protein electrophoresis (SPE), measurement of trypsin inhibiting capacity (TIC), isoelectric focusing (IEF), polymerase chain reaction (PCR), and IEF/PCR-RFLP techniques. The result clearly shows by using SPE and TIC techniques only 35.85 % and 50.08% of ...
متن کاملCharacterization of the principal human prostatic acid phosphatase isoenzyme, purified by affinity chromatography and isoelectric focusing. Part II.
The principal enzyme of human prostatic acid phosphatase [orthophosphoric monoester phosphohydrolase (acid optimum), EC 3.1.3.2], which had been highly purified by affinity chromatography, isoelectric focusing, and gel filtrations, was shown to be homogeneous at pH 5.0 by sedimentation equilibrium analysis. The amino acid composition was determined and the sedimentation coefficient of the nativ...
متن کاملIsoenzyme studies on Toxoplasma gondii isolates using isoelectric focusing.
Zymogram analysis using isoelectric focusing on polyacrylamide gels was performed to characterize and distinguish two Toxoplasma gondii isolates ("strains" BK and RH). The activity of the following 14 enzymes in the cell lysates was investigated: IDH, MDH, ME, 6PG, G6P, LDH, IPO, HEX, PGM, EST, ALP, ACP, LAP, and PGI. Nine enzymes (IDH, G6P, LDH, HEX, PGM, EST, ALP, ACP, and PGI) showed distinc...
متن کاملCharacterization of cyclically transmitted Trypanosoma (T.) brucei isolates from man.
Four different trypanosome isolates from human patients isolated in 1979 during the epidemic of sleeping sickness in Busoga, south-east Uganda, were characterized by the following methods: isoenzyme analyses of bloodstream forms by isoelectric focusing; in vitro tests of human serum resistance of bloodstream as well as metacyclic forms; tsetse fly transmission through Glossina morsitans central...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Acta tropica
دوره 40 1 شماره
صفحات -
تاریخ انتشار 1983